target padi2  (Proteintech)

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Immunodetection of PADI2 in various tumor tissues. Notes: PADI2 shows significantly increased expression in many tumor types. “ a ” represents tumor tissue, and “ b ” represents the parallel tumor adjacent tissues or normal tissues. Original magnification: 200×. Abbreviation: PADI2, peptidylarginine deiminase isoform 2.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Immunodetection, Expressing

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Semiquantitation of the PADI2 expression level in various tumor tissues. Notes: The x -axis indicates tumor types and matched normal tissues, and the y -axis indicates the expression level of PADI2. *** P <0.001. Abbreviation: PADI2, peptidylarginine deiminase isoform 2.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Determination of PADI2 levels in the blood samples of patients with tumors using ELISA. Notes: The x -axis represents various tumor types, and the y -axis represents the PADI2 expression level with P/N values. The OD 450 value of the positive serum ( P -value) was divided by the OD 405 value of the pooled negative sera ( N value). The P/N ratio was calculated for each serum sample. *** P <0.001. Abbreviations: PADI2, peptidylarginine deiminase isoform 2; ELISA, enzyme-linked immunosorbent assay; OD, optical density.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Proliferation of MNK-45 cells and Bel-7402 cells treated with anti-PADI2 siRNA. Notes: ( A ) The CCK-8 assay detected MNK-45 viable cell numbers as represented by an OD value at 405 nm. ( B ) The CCK-8 assay detected Bel-7402 viable cell numbers as represented by an OD value at 405 nm. Cells treated with Allstar siRNA were used as negative controls. * P <0.05, ** P <0.01 and *** P <0.001. Abbreviations: PADI2, peptidylarginine deiminase isoform 2; CCK-8, cell counting kit-8; OD, optical density.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: CCK-8 Assay, Cell Counting

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Apoptosis in MNK-45 cells and Bel-7402 cells treated with anti-PADI2 siRNA, as measured using the annexin V cell apoptosis assay. Notes: ( A ) MNK-45 cells were treated with anti-PADI2 siRNA. ( B ) MNK-45 cells treated with Allstar siRNA were used as negative controls. ( C ) Result of the apoptosis assay for MNK-45 cells. ( D ) Bel-7402 cells were treated with anti-PADI2 siRNA. ( E ) Bel-7402 cells treated with Allstar siRNA were used as negative controls. ( F ) Result of the apoptosis assay for MNK-45 cells. *** P <0.05. Abbreviation: PADI2, peptidylarginine deiminase isoform 2.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Apoptosis Assay

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Migration of MNK-45 and Bel-7402 cells treated with anti-PADI2 siRNA, as measured by the transwell migration assay. Notes: ( A ) MNK-45 cells were treated with anti-PADI2 siRNA. ( B ) MNK-45 cells treated with Allstar siRNA were used as negative controls. ( C ) Result of the migration measurement for MNK-45 cells. ( D ) Bel-7402 cells were treated with anti-PADI2 siRNA. ( E ) Bel-7402 cells treated with Allstar siRNA were used as negative controls. ( F ) Result of the migration measurement for Bel-7402 cells. Original magnification: 4.2×. *** P <0.001. Abbreviation: PADI2, peptidylarginine deiminase isoform 2.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Migration, Transwell Migration Assay

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Determination of significantly altered gene expression using PCR arrays. Notes: Tumor cell lines MNK-45 and Bel-7402 were treated with anti-PADI2 siRNA. Cells treated with Allstar siRNA were used as a negative control. Significantly altered expression of tumor-related genes was detected using ( A ) Cancer PathwayFinder PCR array, ( B ) Signal Transduction PCR array, ( C ) Tumor Metastasis PCR array, ( D ) Oncogenes and Tumor Suppressor Genes PCR array and ( E ) p53 Signaling PCR array. Abbreviations: PCR, polymerase chain reaction; PADI2, peptidylarginine deiminase isoform 2.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Expressing, Negative Control, Transduction, Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: Investigating the expression, effect and tumorigenic pathway of PADI2 in tumors

doi: 10.2147/OTT.S92389

Figure Lengend Snippet: Determination of the mRNA expression levels of CXCR2 and EPO using real-time PCR. Notes: MNK-45 cells and Bel-7402 cells were treated with anti-PADI2 siRNA. The transcription levels of the target genes in Allstar siRNA-treated cells were set as 1. The target genes in the anti-PADI2 siRNA-treated cells were normalized to their expression in the controls. ( A ) CXCR2 expression level in MNK-45 cells and ( B ) EPO expression level in Bel-7402 cells. *** P <0.001. Abbreviations: PCR, polymerase chain reaction; PADI2, peptidylarginine deiminase isoform 2.

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene (target sequence: 5′-CCCGTTCTTCGGCCAACGCTA-3′) were commercially obtained from Qiagen (Duesseldorf, Germany).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

Journal: Arthritis Research & Therapy

Article Title: Effect of Porphyromonas gingivalis infection on gut dysbiosis and resultant arthritis exacerbation in mouse model

doi: 10.1186/s13075-020-02348-z

Figure Lengend Snippet: Effect of PgPAD on the onset of joint arthritis. The effect of PgPAD on the onset of arthritis was determined. In this experiment, PgPAD deletion mutant 33277 strain (⊿ pad ) and Pg 33277 wild-type (WT) strain were used, unless Pg W83 strain was specifically described in caption of Figures. ABL of the upper jaw in each group was measured ( a ). Ctrl, PBS inoculation; WT, Pg wild-type inoculation; ⊿ pad , Pg pad knockout strain inoculation. To evaluate inflammation of the gingival tissue 6 weeks after Pg inoculation, IL-6 production in gingival tissue of SKG mice was measured by ELISA ( b ). AS was measured 3 and 6 weeks after Pg inoculation with LA i.p. injection ( c ). To assess CP synthesis, ELISA was performed ( d gingival tissue, e joint tissue, f small intestine, g large intestine). The titer of serum ACPA 6 weeks after Pg inoculation with LA i.p. injection in mice from each group was measured by ELISA ( h ). The production of endogenous PADI2 and PADI4 in joint tissue was determined by Western blotting, and then the density of each band was measured by NIH Image-J ( i and j ). Data represent mean ± SD ( b – j ) of 5 mice per group. Statistical analyses were performed using the Tukey-Kramer test and Bonferroni-corrected Mann-Whitney U test for multiple comparisons (* P < 0.05, ** P < 0.01)

Article Snippet: As a control, the amount of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by anti-GAPDH antibody (10 μg/ml, HRP-60004, PROTEINTECH JAPAN, Japan) The density of target PADI2 and PADI4 bands was measured by NIH Image-J software.

Techniques: Mutagenesis, Knock-Out, Enzyme-linked Immunosorbent Assay, Injection, Western Blot, MANN-WHITNEY

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: PADI2 was upregulated in ovarian cancer samples and high PADI2 expression was correlated with poor outcome. a Analysis of PADI2 expression level in human OC. Gene Expression Profiling Interactive Analysis (GEPIA) showed that the red and gray boxes represent cancer and normal tissues, respectively. b Box plots derived from gene expression data in Oncomine comparing expression of PADI2 gene in normal tissue (left plot) and ovarian cancer tissue (right plot). c Kaplan–Meier survival statistics analysis for the relationship between survival time and PADI2 signature in ovarian cancer was performed by using the online tool ( https://kmplot.com/analysis/ ).* P < 0.05, ** P < 0.01 and *** P < 0.001 compared with control group

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Expressing, Gene Expression, Derivative Assay, Control

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Stable knockdown of PADI2 in ovarian cancer cell lines after 72 h lentivirus infection and its effect on the expression of translator-level proteins. a Ovarian cancer cells in the bright field are at the left and fluorescence cells in the same field are at the right. Green fluorescence showed the cells that had been successfully transfected with lentivirus. It could be seen from the figure that infection abundance was high. The scale is 500 μm. b Western blot was used to verify the changes in the translation level of PADI2. GAPDH was used as the internal control. All data were expressed as the mean ± standard deviation of three repeated experiment values. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Knockdown, Infection, Expressing, Fluorescence, Transfection, Western Blot, Control, Standard Deviation

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulating PADI2 suppresses colony formation and proliferation of A2780 and SKOV3 cells. a PADI2 knockdown inhibited A2780 and SKOV3 cell number of colony formation. b MTT assay examined the proliferation ability of A2780 and SKOV3 cells after PADI2 knockdown. c EdU proliferation of A2780 and SKOV3 cells after PADI2 knockdown. A2780 and SKOV3 cells were treated as indicated and stained for EdU incorporation (green) or DAPI (blue) to highlight nuclei(Scale bar 25 μm). EdU positive A2780 and SKOV3 cells ratio was shown on the right side. All data are expressed as the mean ± SD of values from triplicate experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control group

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Knockdown, MTT Assay, Staining, Control

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulating PADI2 suppresses the migration and invasion of A2780 and SKOV3 cells. a Wound-healing assay was performed in A2780 and SKOV3 cells(Scale bar 100 μm). b Cell migration and migrated number were showed by transwell assay in A2780 and SKOV3 cells after PADI2 knockdown (Scale bar 25 μm). Quantitative analyses of migration and invasion abilities showed in the right side. All data are expressed as the mean ± SD of values from triplicate experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 compared with control group

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Migration, Wound Healing Assay, Transwell Assay, Knockdown, Control

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulating PADI2 and Olaparib combination treatment attenuated the viability of ovarian cancer cells. a PADI2 knockdown inhibited A2780 and SKOV3 cell number of colony formation with and without the treatment with Olaparib. b MTT assay examined the proliferation ability of A2780 and SKOV3 cells after PADI2 knockdown with and without the treatment with Olaparib. c EdU proliferation of A2780 and SKOV3 cells after PADI2 knockdown with and without the treatment with Olaparib. A2780 and SKOV3 cells were treated as indicated and stained for EdU incorporation (green) or DAPI (blue) to highlight nuclei(Scale bar 25 μm). EdU positive A2780 and SKOV3 cells ratio was shown on the right side. All data are expressed as the mean ± SD of values from triplicate experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 compared with control group

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Knockdown, MTT Assay, Staining, Control

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulating PADI2 and Olaparib combination treatment suppresses the migration and invasion of A2780 and SKOV3 cells. a Wound-healing assay was performed in A2780 and SKOV3 cells(Scale bar 100 μm). b Cell migration and migrated number were showed by transwell assay in A2780 and SKOV3 cells after PADI2 knockdown with and without the treatment with Olaparib(Scale bar 25 μm). Quantitative analyses of migration and invasion abilities showed in the right side. All data are expressed as the mean ± SD of values from triplicate experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 compared with control group

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Migration, Wound Healing Assay, Transwell Assay, Knockdown, Control

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulating PADI2 and Olaparib combination treatment suppresses EMT and JAK2/STAT3 signaling pathway in A2780 and SKOV3 cells. Western blotting analyses of SKOV3 and A2780 cells treated as described above. E-Cadherin, Claudin-1, Vimentin, ZEB1, N-Cadherin, phospho-Stat3 (Tyr705), phospho-Jak2 (Tyr1007), t-Stat3 and t-Jak2 levels were carried out. GAPDH served as a loading control. Fold changes of the proteins were shown on the bottom. All data are expressed as the mean ± SD of values from triplicate experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 compared with control group

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: Western Blot, Control

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulation of PADI2 combinated with Olaparib repressed the proliferation of tumor cells in vivo. a SKOV3 stable cell line was used to construct ovarian cancer subcutaneous xenograft model of nude mouse. The body weight of each mouse was measured every three days since the indicated treatments began. n = 5. b The nodules were lighter in SKOV3-shPADI2 combinated with Olaparib group compared with the control group. Quantification of tumors weight is on the right. c Tumors grew more slowly after transfection of PADI2 shRNA in vivo. Quantitative analyses of tumors growth. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: In Vivo, Stable Transfection, Construct, Control, Transfection, shRNA

Journal: Journal of Translational Medicine

Article Title: Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib

doi: 10.1186/s12967-020-02528-0

Figure Lengend Snippet: Downregulation of PADI2 combinated with Olaparib inhibited epithelial-mesenchymal transition and STAT3 of tumor in vivo. a Representative IHC staining showed the expression of PADI2 E-cadherin, Vimentin and phospho-STAT3 in tumor issues (× 200 magnification, scale:100 μm). b Quantification of the expression of PADI2 E-cadherin, Vimentin and phospho-STAT3. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The siRNA oligonucleotides targeting the PADI2 gene were commercially obtained from Shanghai GenePharma Co., Ltd. A2780 and SKOV3 cells were transfected with the anti-PADI2 siRNAs using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h. The inhibition of PADI2 expression in these cell lines was verified using Western blot.

Techniques: In Vivo, Immunohistochemistry, Expressing